•         PCR (including primer design)

•         Gateway BP and LR reactions and additional cloning techniques

•         Growth of E.coli cells

•         Plasmid preparation (minipreps)

•         Restriction digestion (single and double enzyme digestion)

•         SAP and CIP treatment

•         Klenow treatment

•         Ligation

•         Transformation of plasmids into competent E.coli cells

•         Cell growth monitoring for E.coli by OD (600nm) and protein expression by fluorescence (GFP)

•         Expression culture induction

•         Cell lysis with detergent

•         Separation of soluble and insoluble E.coli cell lysis fractions by centrifugation

•         Sample preparation for SDS PAGE analysis

•         Solubilisation of insoluble inclusion bodies

•         Purification of proteins from fractions under native or denaturing conditions

•         On-column refolding and purification of denatured proteins on a nickel affinity matrix

•         Preparation of transposed baculovirus DNA for use in insect expression cultures (BAC to BAC system)